› 1udw

X-ray diffraction
2.6Å resolution

Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, CTP

Released:
DOI: 10.2210/pdb1udw/pdb
PDB entry 1udw coloured by chain, front view.
PDB entry 1udw coloured by chain, side view.
PDB entry 1udw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase.
Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F
Structure 12 751-64 (2004)
PMID: 15130468

Function and Biology Details

Reaction catalysed: 
ATP + cytidine = ADP + CMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine-cytidine kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BZX2 (Residues: 1-250; Coverage: 96%)
Gene names: UCK2, UMPK
Sequence domains: Phosphoribulokinase / Uridine kinase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand CTP 2 x CTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: F222
Unit cell:
a: 133.204Å b: 248.097Å c: 91.927Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.21 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Regulation of mammalian nucleotide metabolism and biosynthesis.
Lane et al. (2015)
3 more

3 mentions without citation

In Silico Discovery of Potential Uridine-Cytidine Kinase 2 Inhibitors from the Rhizome of Alpinia mutica.
Malami et al. (2016)
2 more