› 1uec

X-ray diffraction
1.82Å resolution

Crystal structure of autoinhibited form of tandem SH3 domain of p47phox

Released:
DOI: 10.2210/pdb1uec/pdb
PDB entry 1uec coloured by chain, front view.
PDB entry 1uec coloured by chain, side view.
PDB entry 1uec coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A molecular mechanism for autoinhibition of the tandem SH3 domains of p47phox, the regulatory subunit of the phagocyte NADPH oxidase.
Yuzawa S, Suzuki NN, Fujioka Y, Ogura K, Sumimoto H, Inagaki F
Genes Cells 9 443-56 (2004)
PMID: 15147273

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-146972 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil cytosol factor 1 Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 22.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P14598 (Residues: 150-340; Coverage: 49%)
Gene names: NCF1, NOXO2, SH3PXD1A
Sequence domains:
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P41212
Unit cell:
a: 100.217Å b: 100.217Å c: 44.924Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.236
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Combating oxidative stress in vascular disease: NADPH oxidases as therapeutic targets.
Drummond et al. (2011)
9 more

4 mentions without citation

Activation and assembly of the NADPH oxidase: a structural perspective.
Groemping et al. (2005)
3 more