› 1uj3

X-ray diffraction
2.1Å resolution

Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor

Released:
DOI: 10.2210/pdb1uj3/pdb
PDB entry 1uj3 coloured by chain, front view.
PDB entry 1uj3 coloured by chain, side view.
PDB entry 1uj3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a humanized Fab fragment of anti-tissue-factor antibody in complex with tissue factor.
Ohto U, Mizutani R, Nakamura M, Adachi H, Satow Y
J Synchrotron Radiat 11 105-8 (2004)
PMID: 14646147

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233287 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
IgG Fab light chain Chain: A
Molecule details ›
Chain: A
Length: 215 amino acids
Theoretical weight: 23.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
IgG Fab heavy chain Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Tissue factor Chain: C
Molecule details ›
Chain: C
Length: 205 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 38-242; Coverage: 78%)
Gene name: F3
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 103.054Å b: 265.996Å c: 42.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.227
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Tissue factor around dermal vessels has bound factor VII in the absence of injury.
Hoffman et al. (2007)
3 more

7 mentions without citation

Blind prediction performance of RosettaAntibody 3.0: grafting, relaxation, kinematic loop modeling, and full CDR optimization.
Weitzner et al. (2014)
6 more