› 1unq

X-ray diffraction
0.98Å resolution

High resolution crystal structure of the Pleckstrin Homology Domain Of Protein Kinase B/Akt Bound To Ins(1,3,4,5)-Tetrakisphophate

Released:
DOI: 10.2210/pdb1unq/pdb
PDB entry 1unq coloured by chain, front view.
PDB entry 1unq coloured by chain, side view.
PDB entry 1unq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding of phosphatidylinositol 3,4,5-trisphosphate to the pleckstrin homology domain of protein kinase B induces a conformational change.
Milburn CC, Deak M, Kelly SM, Price NC, Alessi DR, Van Aalten DM
Biochem J 375 531-8 (2003)
PMID: 12964941

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152081 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAC-alpha serine/threonine-protein kinase Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31749 (Residues: 1-123; Coverage: 26%)
Gene names: AKT1, PKB, RAC
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand 4IP 1 x 4IP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 82.904Å b: 34.388Å c: 44.292Å
α: 90° β: 115.29° γ: 90°
R-values:
R R work R free
0.151 not available 0.179
Expression system: Escherichia coli BL21

Quick links

Citations

45 review citations

PDK1, the master regulator of AGC kinase signal transduction.
Mora et al. (2004)
44 more

106 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
105 more