› 1urn

X-ray diffraction
1.92Å resolution

U1A MUTANT/RNA COMPLEX + GLYCEROL

Released:
DOI: 10.2210/pdb1urn/pdb
PDB entry 1urn coloured by chain, front view.
PDB entry 1urn coloured by chain, side view.
PDB entry 1urn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.
Oubridge C, Ito N, Evans PR, Teo CH, Nagai K
Nature 372 432-8 (1994)
PMID: 7984237

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140469 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 97 amino acids
Theoretical weight: 11.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 2-98; Coverage: 34%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
RNA (5'-R(*AP*AP*UP*CP*CP*AP*UP*UP*GP*CP*AP*CP*UP*CP*CP*GP*G P*AP*UP*UP*U)-3') Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 21 nucleotides
Theoretical weight: 6.61 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: P6522
Unit cell:
a: 97Å b: 97Å c: 255.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.207 not available
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

90 review citations

Spliceosome structure and function.
Will et al. (2011)
89 more

81 mentions without citation

RNA-binding proteins: modular design for efficient function.
Lunde et al. (2007)
80 more