› 1uu9

X-ray diffraction
1.95Å resolution

Structure of human PDK1 kinase domain in complex with BIM-3

Released:
DOI: 10.2210/pdb1uu9/pdb
PDB entry 1uu9 coloured by chain, front view.
PDB entry 1uu9 coloured by chain, side view.
PDB entry 1uu9 coloured by chain, top view.
Source organism: Spodoptera frugiperda
Primary publication:
Interactions of LY333531 and other bisindolyl maleimide inhibitors with PDK1.
Komander D, Kular GS, Schüttelkopf AW, Deak M, Prakash KR, Bain J, Elliott M, Garrido-Franco M, Kozikowski AP, Alessi DR, van Aalten DM
Structure 12 215-26 (2004)
PMID: 14962382

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-phosphoinositide-dependent protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 33.14 KDa
Source organism: Spodoptera frugiperda
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O15530 (Residues: 72-357; Coverage: 51%)
Gene names: PDK1, PDPK1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 8 x GOL
Ligand SO4 5 x SO4
Ligand BI3 1 x BI3
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P3221
Unit cell:
a: 121.629Å b: 121.629Å c: 48.018Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.183 0.224
Expression system: Spodoptera frugiperda

Quick links

Citations

7 review citations

Indolocarbazole natural products: occurrence, biosynthesis, and biological activity.
Sánchez et al. (2006)
6 more

5 mentions without citation

Bisindolyl Maleimides and Indolylmaleimide Derivatives-A Review of Their Synthesis and Bioactivity.
Cooney et al. (2023)
4 more