› 1uw5

X-ray diffraction
2.9Å resolution

Structure of PITP-alpha complexed to phosphatidylinositol

Released:
DOI: 10.2210/pdb1uw5/pdb
PDB entry 1uw5 coloured by chain, front view.
PDB entry 1uw5 coloured by chain, side view.
PDB entry 1uw5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-function analysis of human [corrected] phosphatidylinositol transfer protein alpha bound to phosphatidylinositol.
Tilley SJ, Skippen A, Murray-Rust J, Swigart PM, Stewart A, Morgan CP, Cockcroft S, McDonald NQ
Structure 12 317-26 (2004)
PMID: 14962392

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169398 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphatidylinositol transfer protein alpha isoform Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 270 amino acids
Theoretical weight: 32.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00169 (Residues: 1-270; Coverage: 100%)
Gene names: PITPN, PITPNA
Sequence domains: Phosphatidylinositol transfer protein
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4

Ligands and Environments

1 bound ligand:
Ligand PIE 4 x PIE
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P212121
Unit cell:
a: 83.262Å b: 93.173Å c: 161.732Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.237 0.307
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
23 more

10 mentions without citation

Inositol lipid regulation of lipid transfer in specialized membrane domains.
Kim et al. (2013)
9 more