› 1v41

X-ray diffraction
2.85Å resolution

Crystal structure of human PNP complexed with 8-Azaguanine

Released:
DOI: 10.2210/pdb1v41/pdb
PDB entry 1v41 coloured by chain, front view.
PDB entry 1v41 coloured by chain, side view.
PDB entry 1v41 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of human PNP complexed with ligands.
Canduri F, Silva RG, dos Santos DM, Palma MS, Basso LA, Santos DS, de Azevedo WF
Acta Crystallogr D Biol Crystallogr 61 856-62 (2005)
PMID: 15983407

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: E
Molecule details ›
Chain: E
Length: 288 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 2-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand AZG 1 x AZG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: R32
Unit cell:
a: 139.28Å b: 139.28Å c: 161.42Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.186 0.263
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Targeting purine and pyrimidine metabolism in human apicomplexan parasites.
Hyde JE. (2007)
2 more

1 mention without citation

Design and directed evolution of a dideoxy purine nucleoside phosphorylase.
Nannemann et al. (2010)