› 1v7m

X-ray diffraction
2.51Å resolution

Human Thrombopoietin Functional Domain Complexed To Neutralizing Antibody TN1 Fab

Released:
DOI: 10.2210/pdb1v7m/pdb
PDB entry 1v7m coloured by chain, front view.
PDB entry 1v7m coloured by chain, side view.
PDB entry 1v7m coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the receptor-binding domain of human thrombopoietin determined by complexation with a neutralizing antibody fragment.
Feese MD, Tamada T, Kato Y, Maeda Y, Hirose M, Matsukura Y, Shigematsu H, Muto T, Matsumoto A, Watarai H, Ogami K, Tahara T, Kato T, Miyazaki H, Kuroki R
Proc Natl Acad Sci U S A 101 1816-21 (2004)
PMID: 14769915

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-233305 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Monoclonal TN1 Fab Light Chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 213 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Monoclonal TN1 Fab Heavy Chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 217 amino acids
Theoretical weight: 23.36 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
Thrombopoietin Chains: V, X
Molecule details ›
Chains: V, X
Length: 163 amino acids
Theoretical weight: 17.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P40225 (Residues: 22-184; Coverage: 49%)
Gene names: MGDF, THPO
Sequence domains: Erythropoietin/thrombopoietin
Structure domains: Growth Hormone; Chain: A;

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 132.099Å b: 46.771Å c: 185.151Å
α: 90° β: 90.48° γ: 90°
R-values:
R R work R free
0.232 0.228 0.316
Expression systems:
  • Mus musculus
  • Escherichia coli

Quick links

Citations

16 review citations

New thrombopoietic growth factors.
Kuter DJ. (2007)
15 more

15 mentions without citation

The thrombopoietin receptor: revisiting the master regulator of platelet production.
Hitchcock et al. (2021)
14 more