› 1w2c

X-ray diffraction
1.95Å resolution

Human Inositol (1,4,5) trisphosphate 3-kinase complexed with Mn2+/AMPPNP/Ins(1,4,5)P3

Released:
DOI: 10.2210/pdb1w2c/pdb
PDB entry 1w2c coloured by chain, front view.
PDB entry 1w2c coloured by chain, side view.
PDB entry 1w2c coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a human inositol 1,4,5-trisphosphate 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinase.
González B, Schell MJ, Letcher AJ, Veprintsev DB, Irvine RF, Williams RL
Mol Cell 15 689-701 (2004)
PMID: 15350214

Function and Biology Details

Reaction catalysed: 
ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-trisphosphate 3-kinase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 30.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23677 (Residues: 197-461; Coverage: 58%)
Gene name: ITPKA
Sequence domains: Inositol polyphosphate kinase
Structure domains: Inositol polyphosphate kinase

Ligands and Environments

4 bound ligands:
Ligand ANP 2 x ANP
Ligand I3P 2 x I3P
Ligand MN 2 x MN
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 72.063Å b: 97.517Å c: 191.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.199 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

14 review citations

Inositol polyphosphates: a new frontier for regulating gene expression.
Alcázar-Román et al. (2008)
13 more

13 mentions without citation

The enzymes of human diphosphoinositol polyphosphate metabolism.
Thomas et al. (2014)
12 more