› 1w2f

X-ray diffraction
1.8Å resolution

Human Inositol (1,4,5)-trisphosphate 3-kinase substituted with selenomethionine

Released:
DOI: 10.2210/pdb1w2f/pdb
PDB entry 1w2f coloured by chain, front view.
PDB entry 1w2f coloured by chain, side view.
PDB entry 1w2f coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a human inositol 1,4,5-trisphosphate 3-kinase: substrate binding reveals why it is not a phosphoinositide 3-kinase.
González B, Schell MJ, Letcher AJ, Veprintsev DB, Irvine RF, Williams RL
Mol Cell 15 689-701 (2004)
PMID: 15350214

Function and Biology Details

Reaction catalysed: 
ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-trisphosphate 3-kinase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 276 amino acids
Theoretical weight: 31.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P23677 (Residues: 186-461; Coverage: 60%)
Gene name: ITPKA
Sequence domains: Inositol polyphosphate kinase
Structure domains: Inositol polyphosphate kinase

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2221
Unit cell:
a: 69.304Å b: 95.798Å c: 180.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.241
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Inositol polyphosphates: a new frontier for regulating gene expression.
Alcázar-Román et al. (2008)
14 more

3 mentions without citation

The enzymes of human diphosphoinositol polyphosphate metabolism.
Thomas et al. (2014)
2 more