› 1w6p

X-ray diffraction
1.8Å resolution

X-RAY CRYSTAL STRUCTURE OF C2S HUMAN GALECTIN-1 COMPLEXED WITH N- Acetyl-LACTOSAMINE

Released:
DOI: 10.2210/pdb1w6p/pdb
PDB entry 1w6p coloured by chain, front view.
PDB entry 1w6p coloured by chain, side view.
PDB entry 1w6p coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Growth-regulatory human galectin-1: crystallographic characterisation of the structural changes induced by single-site mutations and their impact on the thermodynamics of ligand binding.
López-Lucendo MF, Solís D, André S, Hirabayashi J, Kasai K, Kaltner H, Gabius HJ, Romero A
J Mol Biol 343 957-70 (2004)
PMID: 15476813

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140623 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Galectin-1 Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 14.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09382 (Residues: 2-135; Coverage: 99%)
Gene name: LGALS1
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls
Galectin-1 Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09382 (Residues: 2-135; Coverage: 99%)
Gene name: LGALS1
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, GAL
Carbohydrate polymer
2 bound ligands:
Ligand BME 5 x BME
Ligand SO4 1 x SO4
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 36.76Å b: 88.1Å c: 93.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.238
Expression system: Escherichia coli

Quick links

Citations

28 review citations

Galectin-1: a small protein with major functions.
Camby et al. (2006)
27 more

10 mentions without citation

Dissecting the Structure-Activity Relationship of Galectin-Ligand Interactions.
Chan et al. (2018)
9 more