› 1war

X-ray diffraction
2.22Å resolution

Recombinant Human Purple Acid Phosphatase expressed in Pichia Pastoris

Released:
DOI: 10.2210/pdb1war/pdb
PDB entry 1war coloured by chain, front view.
PDB entry 1war coloured by chain, side view.
PDB entry 1war coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of recombinant human purple Acid phosphatase with and without an inhibitory conformation of the repression loop.
Sträter N, Jasper B, Scholte M, Krebs B, Duff AP, Langley DB, Han R, Averill BA, Freeman HC, Guss JM
J Mol Biol 351 233-46 (2005)
PMID: 15993892

Function and Biology Details

Reaction catalysed: 
A phosphate monoester + H(2)O = an alcohol + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146697 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tartrate-resistant acid phosphatase type 5 Chain: A
Molecule details ›
Chain: A
Length: 310 amino acids
Theoretical weight: 35.05 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P13686 (Residues: 19-325; Coverage: 100%)
Gene name: ACP5
Sequence domains: Calcineurin-like phosphoesterase
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

3 bound ligands:
Ligand FE 2 x FE
Ligand PO4 1 x PO4
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 80.538Å b: 80.538Å c: 99.998Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.141 0.138 0.198
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Metallophosphoesterases: structural fidelity with functional promiscuity.
Matange et al. (2015)
5 more

7 mentions without citation

Crystal structure of GAP50, the anchor of the invasion machinery in the inner membrane complex of Plasmodium falciparum.
Bosch et al. (2012)
6 more