› 1wl5

X-ray diffraction
2.26Å resolution

Human cytosolic acetoacetyl-CoA thiolase

Released:
DOI: 10.2210/pdb1wl5/pdb
PDB entry 1wl5 coloured by chain, front view.
PDB entry 1wl5 coloured by chain, side view.
PDB entry 1wl5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High resolution crystal structures of human cytosolic thiolase (CT): a comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I.
Kursula P, Sikkilä H, Fukao T, Kondo N, Wierenga RK
J Mol Biol 347 189-201 (2005)
PMID: 15733928

Function and Biology Details

Reaction catalysed: 
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189774 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase, cytosolic Chain: A
Molecule details ›
Chain: A
Length: 397 amino acids
Theoretical weight: 41.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BWD1 (Residues: 1-397; Coverage: 100%)
Gene names: ACAT2, ACTL
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand GOL 2 x GOL
1 modified residue:
Ligand CSO 1 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: I222
Unit cell:
a: 57.85Å b: 109.88Å c: 155.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The thiolase superfamily: condensing enzymes with diverse reaction specificities.
Haapalainen et al. (2006)
26 other articles

3 mentions without citation

A structural view of ligand-dependent activation in thermoTRP channels.
Steinberg et al. (2014)
2 more