› 1wt5

X-ray diffraction
2.1Å resolution

The Crystal Structure Of A Humanized Antibody Fv 528

Released:
DOI: 10.2210/pdb1wt5/pdb
PDB entry 1wt5 coloured by chain, front view.
PDB entry 1wt5 coloured by chain, side view.
PDB entry 1wt5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Thermodynamic consequences of mutations in vernier zone residues of a humanized anti-human epidermal growth factor receptor murine antibody, 528.
Makabe K, Nakanishi T, Tsumoto K, Tanaka Y, Kondo H, Umetsu M, Sone Y, Asano R, Kumagai I
J Biol Chem 283 1156-66 (2008)
PMID: 17947238

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213109 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ANTI EGFR ANTIBODY FV REGION Chains: A, B
Molecule details ›
Chains: A, B
Length: 127 amino acids
Theoretical weight: 14.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins
ANTI EGFR ANTIBODY FV REGION Chains: C, D
Molecule details ›
Chains: C, D
Length: 122 amino acids
Theoretical weight: 13.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P65
Unit cell:
a: 63.282Å b: 63.282Å c: 225.339Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.273 0.273 0.301
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Antibody humanization methods - a review and update.
Safdari et al. (2013)
8 more

6 mentions without citation

Antibodies as a model system for comparative model refinement.
Sellers et al. (2010)
5 more