› 1wzv

X-ray diffraction
2.1Å resolution

Crystal Structure of UbcH8

Released:
DOI: 10.2210/pdb1wzv/pdb
PDB entry 1wzv coloured by chain, front view.
PDB entry 1wzv coloured by chain, side view.
PDB entry 1wzv coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Mizushima T, Suzuki M, Teshima N, Yamane T, Murata S, Tanaka K

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127206 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin/ISG15-conjugating enzyme E2 L6 Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 17.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14933 (Residues: 2-153; Coverage: 99%)
Gene names: UBCH8, UBE2L6
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P21
Unit cell:
a: 54.231Å b: 35.486Å c: 92.58Å
α: 90° β: 104.55° γ: 90°
R-values:
R R work R free
0.21 0.207 0.267
Expression system: Escherichia coli

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