› 1x8u

X-ray diffraction
2.2Å resolution

Crystal structure of Siderocalin (NGAL, Lipocalin 2) complexed with Carboxymycobactin T

Released:
DOI: 10.2210/pdb1x8u/pdb
PDB entry 1x8u coloured by chain, front view.
PDB entry 1x8u coloured by chain, side view.
PDB entry 1x8u coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Siderocalin (Lcn 2) also binds carboxymycobactins, potentially defending against mycobacterial infections through iron sequestration.
Holmes MA, Paulsene W, Jide X, Ratledge C, Strong RK
Structure 13 29-41 (2005)
PMID: 15642259

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160356 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil gelatinase-associated lipocalin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 178 amino acids
Theoretical weight: 20.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P80188 (Residues: 21-198; Coverage: 100%)
Gene names: HNL, LCN2, NGAL
Sequence domains: Lipocalin / cytosolic fatty-acid binding protein family
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand CM2 3 x CM2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P41212
Unit cell:
a: 114.8Å b: 114.8Å c: 119.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.253
Expression system: Escherichia coli

Quick links

Citations

68 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
67 more

1 mention without citation

Iron traffics in circulation bound to a siderocalin (Ngal)-catechol complex.
Bao et al. (2010)