› 1xcg

X-ray diffraction
2.5Å resolution

Crystal Structure of Human RhoA in complex with DH/PH fragment of PDZRHOGEF

Released:
DOI: 10.2210/pdb1xcg/pdb
PDB entry 1xcg coloured by chain, front view.
PDB entry 1xcg coloured by chain, side view.
PDB entry 1xcg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of RhoA in complex with the DH/PH fragment of PDZRhoGEF, an activator of the Ca(2+) sensitization pathway in smooth muscle.
Derewenda U, Oleksy A, Stevenson AS, Korczynska J, Dauter Z, Somlyo AP, Otlewski J, Somlyo AV, Derewenda ZS
Structure 12 1955-65 (2004)
PMID: 15530360

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127285 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 11 Chains: A, E
Molecule details ›
Chains: A, E
Length: 368 amino acids
Theoretical weight: 42.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15085 (Residues: 714-1081; Coverage: 24%)
Gene names: ARHGEF11, KIAA0380
Sequence domains:
Structure domains:
Transforming protein RhoA Chains: B, F
Molecule details ›
Chains: B, F
Length: 178 amino acids
Theoretical weight: 20.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61586 (Residues: 3-180; Coverage: 92%)
Gene names: ARH12, ARHA, RHO12, RHOA
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 89.236Å b: 118.782Å c: 90.13Å
α: 90° β: 114.34° γ: 90°
R-values:
R R work R free
0.235 0.224 0.281
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Regulation of small GTPases by GEFs, GAPs, and GDIs.
Cherfils et al. (2013)
7 more

19 mentions without citation

Structure and function of heterotrimeric G protein-regulated Rho guanine nucleotide exchange factors.
Aittaleb et al. (2010)
18 more