› 1xf2

X-ray diffraction
2.3Å resolution

Structure of Fab DNA-1 complexed with dT3

Released:
DOI: 10.2210/pdb1xf2/pdb
PDB entry 1xf2 coloured by chain, front view.
PDB entry 1xf2 coloured by chain, side view.
PDB entry 1xf2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition.
Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ
J Mol Biol 347 965-78 (2005)
PMID: 15784256

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
PDBe Complex ID:
PDB-CPX-207782 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct DNA molecule
Macromolecules (3 distinct):
antibody light chain Fab Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
antibody heavy chain Fab Chains: B, H
Molecule details ›
Chains: B, H
Length: 230 amino acids
Theoretical weight: 24.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
5'-D(*TP*TP*T)-3' Chain: T
Molecule details ›
Chain: T
Length: 3 nucleotides
Theoretical weight: 868 Da

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P6522
Unit cell:
a: 170.678Å b: 170.678Å c: 144.667Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.242
Expression system: Escherichia coli

Quick links

Citations

17 citation in other articles

Heavy and light chain variable single domains of an anti-DNA binding antibody hydrolyze both double- and single-stranded DNAs without sequence specificity.
Kim et al. (2006)
16 more