› 1xf4

X-ray diffraction
2.5Å resolution

Structure of ligand-free Fab DNA-1 in space group P321 solved from crystals with perfect hemihedral twinning

Released:
DOI: 10.2210/pdb1xf4/pdb
PDB entry 1xf4 coloured by chain, front view.
PDB entry 1xf4 coloured by chain, side view.
PDB entry 1xf4 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition.
Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ
J Mol Biol 347 965-78 (2005)
PMID: 15784256

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero dodecamer
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-213113 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fab light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.62 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Fab heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 230 amino acids
Theoretical weight: 24.98 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P321
Unit cell:
a: 179.179Å b: 179.179Å c: 91.985Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.183 0.224
Expression system: Escherichia coli

Quick links

Citations

16 citation in other articles

Heavy and light chain variable single domains of an anti-DNA binding antibody hydrolyze both double- and single-stranded DNAs without sequence specificity.
Kim et al. (2006)
15 more

2 mentions without citation

Impact of DNA hairpin folding energetics on antibody-ssDNA association.
Ou et al. (2007)
1 more