› 1xtz

X-ray diffraction
2.1Å resolution

Crystal structure of the S. cerevisiae D-ribose-5-phosphate isomerase: comparison with the archeal and bacterial enzymes

Released:
DOI: 10.2210/pdb1xtz/pdb
PDB entry 1xtz coloured by chain, front view.
PDB entry 1xtz coloured by chain, side view.
PDB entry 1xtz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the S. cerevisiae D-ribose-5-phosphate isomerase: comparison with the archaeal and bacterial enzymes.
Graille M, Meyer P, Leulliot N, Sorel I, Janin J, Van Tilbeurgh H, Quevillon-Cheruel S
Biochimie 87 763-9 (2005)
PMID: 16054529

Function and Biology Details

Reaction catalysed: 
D-ribose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribose-5-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 264 amino acids
Theoretical weight: 29.12 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12189 (Residues: 1-258; Coverage: 100%)
Gene names: RKI1, YOR095C, YOR3174C
Sequence domains: Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: F432
Unit cell:
a: 209.24Å b: 209.24Å c: 209.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.247
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The biochemistry, metabolism and inherited defects of the pentose phosphate pathway: a review.
Wamelink et al. (2008)
1 more

3 mentions without citation

Ribulose-1,5-bisphosphate regeneration in the Calvin-Benson-Bassham cycle: Focus on the last three enzymatic steps that allow the formation of Rubisco substrate.
Meloni et al. (2023)
2 more