› 1y2a

X-ray diffraction
2.2Å resolution

Structure of mammalian importin bound to the non-classical PLSCR1-NLS

Released:
DOI: 10.2210/pdb1y2a/pdb
PDB entry 1y2a coloured by chain, front view.
PDB entry 1y2a coloured by chain, side view.
PDB entry 1y2a coloured by chain, top view.
Source organisms:
Primary publication:
Phospholipid scramblase 1 contains a nonclassical nuclear localization signal with unique binding site in importin alpha.
Chen MH, Ben-Efraim I, Mitrousis G, Walker-Kopp N, Sims PJ, Cingolani G
J Biol Chem 280 10599-606 (2005)
PMID: 15611084

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127336 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Importin subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 428 amino acids
Theoretical weight: 46.39 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52293 (Residues: 70-497; Coverage: 81%)
Gene names: Kpna2, Rch1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant
Phospholipid scramblase 1 Chain: P
Molecule details ›
Chain: P
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O15162 (Residues: 257-266; Coverage: 3%)
Gene name: PLSCR1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 78.239Å b: 91.1Å c: 97.247Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.289 0.229 0.258
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

15 review citations

Structural Biology and Regulation of Protein Import into the Nucleus.
Christie et al. (2016)
14 more

12 mentions without citation

Functional Interfaces, Biological Pathways, and Regulations of Interferon-Related DNA Damage Resistance Signature (IRDS) Genes.
Padariya et al. (2021)
11 more