Function and Biology Details
Reactions catalysed:
ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate
Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Biotin/lipoyl attachment
- Histidine kinase/HSP90-like ATPase
- Histidine kinase/HSP90-like ATPase superfamily
- Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain superfamily
- 2-oxo acid dehydrogenase, lipoyl-binding site
- Dihydrolipoamide acetyltransferase/Pyruvate dehydrogenase protein X component
- Single hybrid motif
- Histidine kinase domain
2 more domains
Structure analysis Details
Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 3, mitochondrial
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 48.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Sequence domains:
Length: 419 amino acids
Theoretical weight: 48.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
Q15120 (Residues: 9-406; Coverage: 98%)
Sequence domains:
- Mitochondrial branched-chain alpha-ketoacid dehydrogenase kinase
- Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Length: 128 amino acids
Theoretical weight: 14.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
- Canonical: P10515 (Residues: 212-319; Coverage: 17%)
Sequence domains: Biotin-requiring enzyme
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
