› 1yag

X-ray diffraction
1.9Å resolution

STRUCTURE OF THE YEAST ACTIN-HUMAN GELSOLIN SEGMENT 1 COMPLEX

Released:
DOI: 10.2210/pdb1yag/pdb
PDB entry 1yag coloured by chain, front view.
PDB entry 1yag coloured by chain, side view.
PDB entry 1yag coloured by chain, top view.
Source organisms:
Primary publication:
The structure of nonvertebrate actin: implications for the ATP hydrolytic mechanism.
Vorobiev S, Strokopytov B, Drubin DG, Frieden C, Ono S, Condeelis J, Rubenstein PA, Almo SC
Proc Natl Acad Sci U S A 100 5760-5 (2003)
PMID: 12732734

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139013 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.74 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P60010 (Residues: 1-375; Coverage: 100%)
Gene names: ABY1, ACT1, END7, YFL039C
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 125 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand ATP 1 x ATP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 176.662Å b: 68.099Å c: 54.598Å
α: 90° β: 102.55° γ: 90°
R-values:
R R work R free
0.193 0.193 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Regulation of actin filament assembly by Arp2/3 complex and formins.
Pollard TD. (2007)
7 more

52 mentions without citation

The evolution of the cytoskeleton.
Wickstead et al. (2011)
51 more