› 1ydk

X-ray diffraction
1.95Å resolution

Crystal structure of the I219A mutant of human glutathione transferase A1-1 with S-hexylglutathione

Released:
DOI: 10.2210/pdb1ydk/pdb
PDB entry 1ydk coloured by chain, front view.
PDB entry 1ydk coloured by chain, side view.
PDB entry 1ydk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Tertiary interactions stabilise the C-terminal region of human glutathione transferase A1-1: a crystallographic and calorimetric study.
Kuhnert DC, Sayed Y, Mosebi S, Sayed M, Sewell T, Dirr HW
J Mol Biol 349 825-38 (2005)
PMID: 15893769

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140083 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase A1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08263 (Residues: 1-222; Coverage: 100%)
Gene name: GSTA1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GTX 2 x GTX
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 99.53Å b: 91.472Å c: 51.482Å
α: 90° β: 92.4° γ: 90°
R-values:
R R work R free
0.205 0.203 0.253
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Interactions of glutathione transferases with 4-hydroxynonenal.
Balogh et al. (2011)
16 other articles

2 mentions without citation

The interaction of the chemotherapeutic drug chlorambucil with human glutathione transferase A1-1: kinetic and structural analysis.
Karpusas et al. (2013)
1 more