› 1yjd

X-ray diffraction
2.7Å resolution

Crystal structure of human CD28 in complex with the Fab fragment of a mitogenic antibody (5.11A1)

Released:
DOI: 10.2210/pdb1yjd/pdb
PDB entry 1yjd coloured by chain, front view.
PDB entry 1yjd coloured by chain, side view.
PDB entry 1yjd coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of a soluble CD28-Fab complex.
Evans EJ, Esnouf RM, Manso-Sancho R, Gilbert RJ, James JR, Yu C, Fennelly JA, Vowles C, Hanke T, Walse B, Hünig T, Sørensen P, Stuart DI, Davis SJ
Nat Immunol 6 271-9 (2005)
PMID: 15696168

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210491 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab fragment of 5.11A1 antibody light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.39 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
Fab fragment of 5.11A1 antibody heavy chain Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 24.11 KDa
Source organism: Mus musculus
Structure domains: Immunoglobulins
T-cell-specific surface glycoprotein CD28 Chain: C
Molecule details ›
Chain: C
Length: 140 amino acids
Theoretical weight: 15.78 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P10747 (Residues: 17-152; Coverage: 66%)
Gene name: CD28
Sequence domains: ICOS V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 3 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: C2
Unit cell:
a: 191.219Å b: 47.417Å c: 71.842Å
α: 90° β: 94.45° γ: 90°
R-values:
R R work R free
0.239 0.239 0.282
Expression system: Cricetulus griseus

Quick links

Citations

28 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
27 more

38 mentions without citation

Sequence, structure, function, immunity: structural genomics of costimulation.
Chattopadhyay et al. (2009)
37 more