› 1ykt

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133397 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Anionic trypsin-2 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.8 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00763 (Residues: 24-246; Coverage: 97%)
Gene names: Prss2, Try2
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Pancreatic trypsin inhibitor Chain: B
Molecule details ›
Chain: B
Length: 56 amino acids
Theoretical weight: 6.4 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00974 (Residues: 36-91; Coverage: 71%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3221
Unit cell:
a: 92.711Å b: 92.711Å c: 62.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.212
Expression system: Escherichia coli

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