Function and Biology Details
Reaction catalysed:
ATP + [NEDD8 protein] + [E1 NEDD8-activating enzyme]-L-cysteine = AMP + diphosphate + [E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- E2 binding
- THIF-type NAD/FAD binding fold
- ThiF/MoeB/HesA family
- Ubiquitin-activating enzyme
- Ubiquitin-activating enzyme E1, Cys active site
- Ubiquitin activating enzyme, alpha domain superfamily
- NEDD8-activating enzyme E1 catalytic subunit, N-terminal domain
- NEDD8-activating enzyme E1 regulatory subunit APP-BP1
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
NEDD8 E1 activating enzyme complex, NAE1-UBA3 (preferred)
PDBe Complex ID:
PDB-CPX-171763 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NEDD8-activating enzyme E1 regulatory subunit
Molecule details ›
Chains: A, C
Length: 537 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
Length: 537 amino acids
Theoretical weight: 60.69 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
Q13564 (Residues: 1-534; Coverage: 100%)
Sequence domains: ThiF family
Structure domains: NAD(P)-binding Rossmann-like Domain
NEDD8-activating enzyme E1 catalytic subunit
Molecule details ›
Chains: B, D
Length: 444 amino acids
Theoretical weight: 49.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains:
Structure domains:
Length: 444 amino acids
Theoretical weight: 49.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical: Q8TBC4 (Residues: 22-463; Coverage: 96%)
Sequence domains:
Structure domains:
