› 1yrp

X-ray diffraction
3.1Å resolution

Catalytic domain of human ZIP kinase phosphorylated at Thr265

Released:
DOI: 10.2210/pdb1yrp/pdb
PDB entry 1yrp coloured by chain, front view.
PDB entry 1yrp coloured by chain, side view.
PDB entry 1yrp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Death-Associated Protein Kinase Activity Is Regulated by Coupled Calcium/Calmodulin Binding to Two Distinct Sites.
Simon B, Huart AS, Temmerman K, Vahokoski J, Mertens HD, Komadina D, Hoffmann JE, Yumerefendi H, Svergun DI, Kursula P, Schultz C, McCarthy AA, Hart DJ, Wilmanns M
Structure 24 851-61 (2016)
PMID: 27133022

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Death-associated protein kinase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 32.06 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43293 (Residues: 1-277; Coverage: 61%)
Gene names: DAPK3, ZIPK
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand DTT 1 x DTT
1 modified residue:
Ligand TPO 2 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P21
Unit cell:
a: 53.56Å b: 60.93Å c: 87.95Å
α: 90° β: 92.17° γ: 90°
R-values:
R R work R free
0.245 0.244 0.268
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Crosstalk of ER stress-mediated autophagy and ER-phagy: Involvement of UPR and the core autophagy machinery.
Song et al. (2018)
7 more

4 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
3 more