› 1ys0

X-ray diffraction
2Å resolution

Crystal Structure of the CDC25B phosphatase catalytic domain with the active site cysteine in the disulfide form

Released:
DOI: 10.2210/pdb1ys0/pdb
PDB entry 1ys0 coloured by chain, front view.
PDB entry 1ys0 coloured by chain, side view.
PDB entry 1ys0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural mechanism of oxidative regulation of the phosphatase Cdc25B via an intramolecular disulfide bond.
Buhrman G, Parker B, Sohn J, Rudolph J, Mattos C
Biochemistry 44 5307-16 (2005)
PMID: 15807524

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
M-phase inducer phosphatase 2 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P30305 (Residues: 391-564; Coverage: 30%)
Gene names: CDC25B, CDC25HU2
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 50.197Å b: 71.156Å c: 73.693Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.209 0.244
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Hydrogen peroxide: a signaling messenger.
Stone et al. (2006)
21 more

2 mentions without citation

Crystal structure of the human lymphoid tyrosine phosphatase catalytic domain: insights into redox regulation .
Tsai et al. (2009)
1 more