› 1yty

X-ray diffraction
2.29Å resolution

Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen

Released:
DOI: 10.2210/pdb1yty/pdb
PDB entry 1yty coloured by chain, front view.
PDB entry 1yty coloured by chain, side view.
PDB entry 1yty coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for recognition and sequestration of UUU(OH) 3' temini of nascent RNA polymerase III transcripts by La, a rheumatic disease autoantigen.
Teplova M, Yuan YR, Phan AT, Malinina L, Ilin S, Teplov A, Patel DJ
Mol Cell 21 75-85 (2006)
PMID: 16387655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138680 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Lupus La protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 194 amino acids
Theoretical weight: 22.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05455 (Residues: 1-194; Coverage: 48%)
Gene name: SSB
Sequence domains:
Structure domains:
5'-R(*UP*GP*CP*UP*GP*UP*UP*UP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 153.69Å b: 54.001Å c: 56.684Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.219 0.288
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

26 review citations

tRNA biology charges to the front.
Phizicky et al. (2010)
25 more

5 mentions without citation

Structural basis for recognition and sequestration of UUU(OH) 3' temini of nascent RNA polymerase III transcripts by La, a rheumatic disease autoantigen.
Teplova et al. (2006)
4 more