› 1yyz

X-ray diffraction
1.85Å resolution

R-State AMP Complex Reveals Initial Steps of the Quaternary Transition of Fructose-1,6-bisphosphatase

Released:
DOI: 10.2210/pdb1yyz/pdb
PDB entry 1yyz coloured by chain, front view.
PDB entry 1yyz coloured by chain, side view.
PDB entry 1yyz coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
R-state AMP complex reveals initial steps of the quaternary transition of fructose-1,6-bisphosphatase.
Iancu CV, Mukund S, Fromm HJ, Honzatko RB
J Biol Chem 280 19737-45 (2005)
PMID: 15767255

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-1,6-bisphosphatase 1 Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 36.73 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00636 (Residues: 2-338; Coverage: 100%)
Gene names: FBP, FBP1
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand F6P 1 x F6P
Ligand PO4 1 x PO4
Ligand MG 2 x MG
Ligand AMP 1 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: I222
Unit cell:
a: 53.78Å b: 82.56Å c: 166.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.266
Expression system: Escherichia coli

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Citations

1 review citation

Structural and mechanistic insights into the bifunctional enzyme isocitrate dehydrogenase kinase/phosphatase AceK.
Zheng et al. (2012)
10 other articles

2 mentions without citation

Crystal structures of human muscle fructose-1,6-bisphosphatase: novel quaternary states, enhanced AMP affinity, and allosteric signal transmission pathway.
Shi et al. (2013)
1 more