› 1z29

X-ray diffraction
2.4Å resolution

Crystal Structures of SULT1A2 and SULT1A1*3: Implications in the bioactivation of N-hydroxy-2-acetylamino fluorine (OH-AAF)

Released:
DOI: 10.2210/pdb1z29/pdb
PDB entry 1z29 coloured by chain, front view.
PDB entry 1z29 coloured by chain, side view.
PDB entry 1z29 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of SULT1A2 and SULT1A1 *3: insights into the substrate inhibition and the role of Tyr149 in SULT1A2.
Lu J, Li H, Zhang J, Li M, Liu MY, An X, Liu MC, Chang W
Biochem Biophys Res Commun 396 429-34 (2010)
PMID: 20417180

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156149 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1A2 Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 34.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P50226 (Residues: 1-295; Coverage: 100%)
Gene names: STP2, SULT1A2
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand A3P 1 x A3P
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2
Unit cell:
a: 58.398Å b: 65.399Å c: 86.517Å
α: 90° β: 103.69° γ: 90°
R-values:
R R work R free
0.243 0.216 0.253
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
4 more

5 mentions without citation

Regioselective sulfation and glucuronidation of phenolics: insights into the structural basis.
Wu et al. (2011)
4 more