› 1zh5

X-ray diffraction
1.85Å resolution

Structural basis for recognition of UUUOH 3'-terminii of nascent RNA pol III transcripts by La autoantigen

Released:
DOI: 10.2210/pdb1zh5/pdb
PDB entry 1zh5 coloured by chain, front view.
PDB entry 1zh5 coloured by chain, side view.
PDB entry 1zh5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for recognition and sequestration of UUU(OH) 3' temini of nascent RNA polymerase III transcripts by La, a rheumatic disease autoantigen.
Teplova M, Yuan YR, Phan AT, Malinina L, Ilin S, Teplov A, Patel DJ
Mol Cell 21 75-85 (2006)
PMID: 16387655

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138681 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Lupus La protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 23.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05455 (Residues: 1-194; Coverage: 48%)
Gene name: SSB
Sequence domains:
Structure domains:
5'-R(*UP*GP*CP*UP*GP*UP*UP*UP*U)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 nucleotides
Theoretical weight: 2.79 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 156.209Å b: 55.03Å c: 57.782Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.2 0.245
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

tRNA biology charges to the front.
Phizicky et al. (2010)
24 more

9 mentions without citation

Towards deciphering the principles underlying an mRNA recognition code.
Serganov et al. (2008)
8 more