› 1zp5

X-ray diffraction
1.8Å resolution

Crystal structure of the complex between MMP-8 and a N-hydroxyurea inhibitor

Released:
DOI: 10.2210/pdb1zp5/pdb
PDB entry 1zp5 coloured by chain, front view.
PDB entry 1zp5 coloured by chain, side view.
PDB entry 1zp5 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
N-Hydroxyurea as zinc binding group in matrix metalloproteinase inhibition: mode of binding in a complex with MMP-8.
Campestre C, Agamennone M, Tortorella P, Preziuso S, Biasone A, Gavuzzo E, Pochetti G, Mazza F, Hiller O, Tschesche H, Consalvi V, Gallina C
Bioorg Med Chem Lett 16 20-4 (2006)
PMID: 16242329

Function and Biology Details

Reaction catalysed: 
Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil collagenase Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P22894 (Residues: 100-262; Coverage: 37%)
Gene names: CLG1, MMP8
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand CA 2 x CA
Ligand ZN 2 x ZN
Ligand 2NI 1 x 2NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P212121
Unit cell:
a: 32.387Å b: 52.675Å c: 67.331Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Matrix metalloproteinase inhibitors as investigative tools in the pathogenesis and management of vascular disease.
Benjamin et al. (2012)
4 more

4 mentions without citation

Zinc-binding groups modulate selective inhibition of MMPs.
Agrawal et al. (2008)
3 more