› 1zvq

X-ray diffraction
2Å resolution

Structure of the Q61G mutant of Ras in the GDP-bound form

Released:
DOI: 10.2210/pdb1zvq/pdb
PDB entry 1zvq coloured by chain, front view.
PDB entry 1zvq coloured by chain, side view.
PDB entry 1zvq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a transient intermediate for GTP hydrolysis by ras.
Ford B, Hornak V, Kleinman H, Nassar N
Structure 14 427-36 (2006)
PMID: 16531227

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 1 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: R32
Unit cell:
a: 92.992Å b: 92.992Å c: 121.015Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.229
Expression system: Escherichia coli

Quick links

Citations

5 review citations

CHARMM: the biomolecular simulation program.
Brooks et al. (2009)
4 more

3 mentions without citation

A structural model of a Ras-Raf signalosome.
Mysore et al. (2021)
2 more