› 1zxn

X-ray diffraction
2.51Å resolution

Human DNA topoisomerase IIa ATPase/ADP

Released:
DOI: 10.2210/pdb1zxn/pdb
PDB entry 1zxn coloured by chain, front view.
PDB entry 1zxn coloured by chain, side view.
PDB entry 1zxn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase.
Wei H, Ruthenburg AJ, Bechis SK, Verdine GL
J Biol Chem 280 37041-7 (2005)
PMID: 16100112

Function and Biology Details

Reaction catalysed: 
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 2-alpha Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 400 amino acids
Theoretical weight: 45.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11388 (Residues: 29-428; Coverage: 26%)
Gene names: TOP2, TOP2A
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 4 x MG
Ligand ADP 4 x ADP
Ligand GOL 1 x GOL
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 71.015Å b: 90.492Å c: 148.293Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.235 0.235 0.281
Expression system: Escherichia coli

Quick links

Citations

15 review citations

DNA topoisomerase II and its growing repertoire of biological functions.
Nitiss JL. (2009)
14 more

13 mentions without citation

A "Double-Edged" Scaffold: Antitumor Power within the Antibacterial Quinolone.
Bisacchi et al. (2016)
12 more