› 1a1j

X-ray diffraction
2Å resolution

RADR (ZIF268 VARIANT) ZINC FINGER-DNA COMPLEX (GCGT SITE)

Released:
DOI: 10.2210/pdb1a1j/pdb
PDB entry 1a1j coloured by chain, front view.
PDB entry 1a1j coloured by chain, side view.
PDB entry 1a1j coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
High-resolution structures of variant Zif268-DNA complexes: implications for understanding zinc finger-DNA recognition.
Elrod-Erickson M, Benson TE, Pabo CO
Structure 6 451-64 (1998)
PMID: 9562555

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113384 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Early growth response protein 1 Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08046 (Residues: 333-421; Coverage: 17%)
Gene names: Egr-1, Egr1, Krox-24
Sequence domains: Zinc finger, C2H2 type
Structure domains: Classic Zinc Finger
DNA (5'-D(*AP*GP*CP*GP*TP*GP*GP*GP*CP*GP*T)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.43 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*TP*AP*CP*GP*CP*CP*CP*AP*CP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.28 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 42.3Å b: 55.9Å c: 133.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.21 0.262
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

DNA recognition by Cys2His2 zinc finger proteins.
Wolfe et al. (2000)
20 more

8 mentions without citation

Zinc-binding cysteines: diverse functions and structural motifs.
Pace et al. (2014)
7 more