› 1a25

X-ray diffraction
2.7Å resolution

C2 DOMAIN FROM PROTEIN KINASE C (BETA)

Released:
DOI: 10.2210/pdb1a25/pdb
PDB entry 1a25 coloured by chain, front view.
PDB entry 1a25 coloured by chain, side view.
PDB entry 1a25 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of the protein kinase Cbeta phospholipid-binding C2 domain complexed with Ca2+.
Sutton RB, Sprang SR
Structure 6 1395-405 (1998)
PMID: 9817842

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase C beta type Chains: A, B
Molecule details ›
Chains: A, B
Length: 149 amino acids
Theoretical weight: 16.88 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68403 (Residues: 154-289; Coverage: 20%)
Gene names: Pkcb, Prkcb, Prkcb1
Sequence domains: C2 domain
Structure domains: C2 domain

Ligands and Environments

2 bound ligands:
Ligand CA 6 x CA
Ligand PSE 1 x PSE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P43212
Unit cell:
a: 77.798Å b: 77.798Å c: 140.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Structural basis of protein kinase C isoform function.
Steinberg SF. (2008)
22 more

15 mentions without citation

Role of Phaseolus vulgaris L. in the Prevention of Cardiovascular Diseases-Cardioprotective Potential of Bioactive Compounds.
Rodríguez et al. (2022)
14 more