› 1a2w

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF A 3D DOMAIN-SWAPPED DIMER OF BOVINE PANCREATIC RIBONUCLEASE A

Released:
DOI: 10.2210/pdb1a2w/pdb
PDB entry 1a2w coloured by chain, front view.
PDB entry 1a2w coloured by chain, side view.
PDB entry 1a2w coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The crystal structure of a 3D domain-swapped dimer of RNase A at a 2.1-A resolution.
Liu Y, Hart PJ, Schlunegger MP, Eisenberg D
Proc Natl Acad Sci U S A 95 3437-42 (1998)
PMID: 9520384

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158404 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P32
Unit cell:
a: 57Å b: 57Å c: 81.41Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.256

Quick links

Citations

17 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
16 more

23 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
22 more