› 1a37

X-ray diffraction
3.6Å resolution

14-3-3 PROTEIN ZETA BOUND TO PS-RAF259 PEPTIDE

Released:
DOI: 10.2210/pdb1a37/pdb
PDB entry 1a37 coloured by chain, front view.
PDB entry 1a37 coloured by chain, side view.
PDB entry 1a37 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
14-3-3zeta binds a phosphorylated Raf peptide and an unphosphorylated peptide via its conserved amphipathic groove.
Petosa C, Masters SC, Bankston LA, Pohl J, Wang B, Fu H, Liddington RC
J Biol Chem 273 16305-10 (1998)
PMID: 9632691

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158964 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63103 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
PS-RAF259 PEPTIDE LSQRQRST(SEP)TPNVHM Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 15 amino acids
Theoretical weight: 1.84 KDa
Source organism: Bos taurus
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P65
Unit cell:
a: 94.73Å b: 94.73Å c: 250.87Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.32 0.32 0.36
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

46 review citations

14-3-3 proteins: structure, function, and regulation.
Fu et al. (2000)
45 more