› 1a65

X-ray diffraction
2.23Å resolution

TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS

Released:
DOI: 10.2210/pdb1a65/pdb
PDB entry 1a65 coloured by chain, front view.
PDB entry 1a65 coloured by chain, side view.
PDB entry 1a65 coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 A resolution.
Ducros V, Brzozowski AM, Wilson KS, Brown SH, Ostergaard P, Schneider P, Yaver DS, Pedersen AH, Davies GJ
Nat Struct Biol 5 310-6 (1998)
PMID: 9546223

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Laccase 1 Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.57 KDa
Source organism: Coprinopsis cinerea
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q9Y780 (Residues: 19-522; Coverage: 97%)
Gene name: lcc1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand PYE 1 x PYE
Ligand CU 3 x CU
Ligand O 1 x O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 45.39Å b: 85.72Å c: 143.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 0.22
Expression system: Aspergillus oryzae

Quick links

Citations

25 review citations

Fungal laccases - occurrence and properties.
Baldrian P. (2006)
24 more

11 mentions without citation

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
10 more