› 1a7l

X-ray diffraction
2.9Å resolution

DOMINANT B-CELL EPITOPE FROM THE PRES2 REGION OF HEPATITIS B VIRUS IN THE FORM OF AN INSERTED PEPTIDE SEGMENT IN MALTODEXTRIN-BINDING PROTEIN

Released:
DOI: 10.2210/pdb1a7l/pdb
PDB entry 1a7l coloured by chain, front view.
PDB entry 1a7l coloured by chain, side view.
PDB entry 1a7l coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Crystal structure of a dominant B-cell epitope from the preS2 region of hepatitis B virus in the form of an inserted peptide segment in maltodextrin-binding protein.
Saul FA, Vulliez-le Normand B, Lema F, Bentley GA
J Mol Biol 280 185-92 (1998)
PMID: 9654443

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-142613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Maltose/maltodextrin-binding periplasmic protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 389 amino acids
Theoretical weight: 42.65 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-389; Coverage: 98%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 64.6Å b: 71.3Å c: 123.2Å
α: 90° β: 94.9° γ: 90°
R-values:
R R work R free
0.192 0.192 0.286
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Design of an expression system to enhance MBP-mediated crystallization.
Jin et al. (2017)
3 more

1 mention without citation

Crystal structure of a defective folding protein.
Saul et al. (2003)