› 1a8s

X-ray diffraction
1.8Å resolution

CHLOROPEROXIDASE F/PROPIONATE COMPLEX

Released:
DOI: 10.2210/pdb1a8s/pdb
PDB entry 1a8s coloured by chain, front view.
PDB entry 1a8s coloured by chain, side view.
PDB entry 1a8s coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Structural investigation of the cofactor-free chloroperoxidases.
Hofmann B, Tölzer S, Pelletier I, Altenbuchner J, van Pée KH, Hecht HJ
J Mol Biol 279 889-900 (1998)
PMID: 9642069

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dodecamer
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-128271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-heme chloroperoxidase Chain: A
Molecule details ›
Chain: A
Length: 273 amino acids
Theoretical weight: 29.55 KDa
Source organism: Pseudomonas fluorescens
Expression system: Escherichia coli
UniProt:
  • Canonical: O31158 (Residues: 2-274; Coverage: 100%)
Gene names: cpo, cpoF
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand PPI 1 x PPI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P23
Unit cell:
a: 106.47Å b: 106.47Å c: 106.47Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.176 0.205
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Alpha/beta hydrolase fold enzymes: the family keeps growing.
Nardini et al. (1999)
17 more

13 mentions without citation

Maspardin is mutated in mast syndrome, a complicated form of hereditary spastic paraplegia associated with dementia.
Simpson et al. (2003)
12 more