› 1ab5

X-ray diffraction
2.4Å resolution

STRUCTURE OF CHEY MUTANT F14N, V21T

Released:
DOI: 10.2210/pdb1ab5/pdb
PDB entry 1ab5 coloured by chain, front view.
PDB entry 1ab5 coloured by chain, side view.
PDB entry 1ab5 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure analysis of two CheY mutants: importance of the hydrogen-bond contribution to protein stability.
Wilcock D, Pisabarro MT, López-Hernandez E, Serrano L, Coll M
Acta Crystallogr D Biol Crystallogr 54 378-85 (1998)
PMID: 9761905

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142405 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chemotaxis protein CheY Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.63 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 5-129; Coverage: 97%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P31
Unit cell:
a: 54.31Å b: 54.31Å c: 91.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.184 0.264
Expression system: Escherichia coli

Quick links

Citations

5 citation in other articles

Understanding hierarchical protein evolution from first principles.
Dokholyan et al. (2001)
4 more

2 mentions without citation

Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.
Klumpler et al. (2009)
1 more