› 1abn

X-ray diffraction
2.4Å resolution

THE CRYSTAL STRUCTURE OF THE ALDOSE REDUCTASE NADPH BINARY COMPLEX

Released:
DOI: 10.2210/pdb1abn/pdb
PDB entry 1abn coloured by chain, front view.
PDB entry 1abn coloured by chain, side view.
PDB entry 1abn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of the aldose reductase.NADPH binary complex.
Borhani DW, Harter TM, Petrash JM
J Biol Chem 267 24841-7 (1992)
PMID: 1447221

Function and Biology Details

Reactions catalysed: 
Alditol + NAD(P)(+) = aldose + NAD(P)H
All-trans-retinol + NADP(+) = all-trans-retinal + NADPH
Glycerol + NADP(+) = D-glyceraldehyde + NADPH
Allyl alcohol + NADP(+) = acrolein + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo-keto reductase family 1 member B1 Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 35.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15121 (Residues: 2-316; Coverage: 100%)
Gene names: AKR1B1, ALDR1, ALR2
Sequence domains: Aldo/keto reductase family

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: I222
Unit cell:
a: 74.71Å b: 85.32Å c: 106.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.286 not available not available
Expression system: Escherichia coli

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Citations

6 review citations

Comparative anatomy of the aldo-keto reductase superfamily.
Jez et al. (1997)
5 more

2 mentions without citation

B-factor Analysis and Conformational Rearrangement of Aldose Reductase.
Balendiran et al. (2014)
1 more