› 1afw

X-ray diffraction
1.8Å resolution

THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERIC PEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE

Released:
DOI: 10.2210/pdb1afw/pdb
PDB entry 1afw coloured by chain, front view.
PDB entry 1afw coloured by chain, side view.
PDB entry 1afw coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The 1.8 A crystal structure of the dimeric peroxisomal 3-ketoacyl-CoA thiolase of Saccharomyces cerevisiae: implications for substrate binding and reaction mechanism.
Mathieu M, Modis Y, Zeelen JP, Engel CK, Abagyan RA, Ahlberg A, Rasmussen B, Lamzin VS, Kunau WH, Wierenga RK
J Mol Biol 273 714-28 (1997)
PMID: 9402066

Function and Biology Details

Reaction catalysed: 
(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-ketoacyl-CoA thiolase, peroxisomal Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 42.08 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P27796 (Residues: 25-417; Coverage: 94%)
Gene names: FOX3, POT1, POX3, YIL160C
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

1 bound ligand:
Ligand MRD 2 x MRD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID2
Spacegroup: P212121
Unit cell:
a: 71.17Å b: 92.65Å c: 116.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.203 0.24

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Citations

6 review citations

The biochemistry of peroxisomal beta-oxidation in the yeast Saccharomyces cerevisiae.
Hiltunen et al. (2003)
5 more

13 mentions without citation

Conservation and relative importance of residues across protein-protein interfaces.
Guharoy et al. (2005)
12 more