› 1aj5

X-ray diffraction
2.3Å resolution

CALPAIN DOMAIN VI APO

Released:
DOI: 10.2210/pdb1aj5/pdb
PDB entry 1aj5 coloured by chain, front view.
PDB entry 1aj5 coloured by chain, side view.
PDB entry 1aj5 coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Structure of a calpain Ca(2+)-binding domain reveals a novel EF-hand and Ca(2+)-induced conformational changes.
Blanchard H, Grochulski P, Li Y, Arthur JS, Davies PL, Elce JS, Cygler M
Nat Struct Biol 4 532-8 (1997)
PMID: 9228945

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179146 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calpain small subunit 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 173 amino acids
Theoretical weight: 20.03 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64537 (Residues: 98-270; Coverage: 64%)
Gene names: Capn4, Capns1, Css1
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 67.5Å b: 73Å c: 156.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.306
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

26 review citations

The calpain system.
Goll et al. (2003)
25 more

6 mentions without citation

Structural and functional diversity of EF-hand proteins: Evolutionary perspectives.
Kawasaki et al. (2017)
5 more