› 1akh

X-ray diffraction
2.5Å resolution

MAT A1/ALPHA2/DNA TERNARY COMPLEX

Released:
DOI: 10.2210/pdb1akh/pdb
PDB entry 1akh coloured by chain, front view.
PDB entry 1akh coloured by chain, side view.
PDB entry 1akh coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the MATa1/MATalpha2 homeodomain heterodimer in complex with DNA containing an A-tract.
Li T, Jin Y, Vershon AK, Wolberger C
Nucleic Acids Res 26 5707-18 (1998)
PMID: 9838003

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113425 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Mating-type protein A1 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 7.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY10 (Residues: 66-126; Coverage: 48%)
Gene names: MAT1A, MATA1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Mating-type protein ALPHA2 Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.77 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CY08 (Residues: 128-210; Coverage: 40%)
Gene names: ALPHA-2, MAT2A, MATAL2, MATALPHA2, YCR039C, YCR39C
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*TP*AP*CP*AP*TP*GP*TP*AP*AP*AP*AP*AP*TP*TP*TP*AP*C P*AP*TP*CP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
DNA (5'-D(*TP*AP*TP*GP*AP*TP*GP*TP*AP*AP*AP*TP*TP*TP*TP*TP*A P*CP*AP*TP*G)-3') Chain: D
Molecule details ›
Chain: D
Length: 21 nucleotides
Theoretical weight: 6.47 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P61
Unit cell:
a: 132.25Å b: 132.25Å c: 45.25Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.302
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
17 other articles

19 mentions without citation

An overview of the structures of protein-DNA complexes.
Luscombe et al. (2000)
18 more