› 1am7

X-ray diffraction
2.3Å resolution

Lysozyme from bacteriophage lambda

Released:
DOI: 10.2210/pdb1am7/pdb
PDB entry 1am7 coloured by chain, front view.
PDB entry 1am7 coloured by chain, side view.
PDB entry 1am7 coloured by chain, top view.
Source organism: Escherichia virus Lambda
Primary publication:
Crystal structure of the lysozyme from bacteriophage lambda and its relationship with V and C-type lysozymes.
Evrard C, Fastrez J, Declercq JP
J Mol Biol 276 151-64 (1998)
PMID: 9514719

Function and Biology Details

Reaction catalysed: 
Endolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137216 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 158 amino acids
Theoretical weight: 17.86 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03706 (Residues: 1-158; Coverage: 100%)
Gene name: R
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:
Ligand IPA 3 x IPA
1 modified residue:
Ligand TRN 12 x TRN

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 73.01Å b: 78.8Å c: 82.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 0.213
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Holins: the protein clocks of bacteriophage infections.
Wang et al. (2000)
2 more

10 mentions without citation

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
9 more